METHOD FOR DESIGNING RECOMBINANT PLASMID DNA PGC S1-39 OF C-TERMINAL AMINOACIDS OF HEPATITIS B VIRUS WITH THE EPITOP P S1 (20-68) EXPOSED ON ITS SURFACE AND BACTERIAL STRAIN OF E COLI AS COR ANTIGEN PRODUCER DEPRIVED OF 39 C-TERMINAL AMINOACIDS OF HEPATITIS B VIRUS WITH THE EPITOP P S1 (20-68) EXPOSED ON ITS SURFACE

摘要

the invention u043eu0442u043du043eu0441u0438u0442u0441u00a0 to the microbiological and medical industry, genetic and protein engineering, and biotechnology.purpose to obtain u0438u0437u043eu0431u0440u0435u0442u0435u043du0438u00a0 u00a0u0432u043bu00a0u0435u0442u0441u00a0 cor - antigen virus hepatitis in man, with u0441u0440u043eu0434u0441u0442u0432u043eu043c to u043du0443u043au043bu0435u0438u043du043eu0432u044bu043c acids, and, in the case a u0445u0438u043cu0435u0440u043du044bu0445 protein structures on the surface of the u044du043au0441u043fu043eu043du0438u0440u0443u044eu0449u0438u0445 cor - antigen from 39 c end amino acid fragment of the preSIHBV x 20 to 68 amino acids.constructed a recombinant gene HBCAg a preSI (20 to 68), encrypting the synthesis of protein, u043fu043bu0430u0437u043cu0438u0434u0430 pGCSI - 140 u0434u043bu00a0 its expression, as well as the strain of e. coli is a producer / pGSI - 140 / re com - u0431u0438u043du0430u043du0442u043du043eu0439 u043au0430u043fu0441u0438u0434u043du043eu0439 structure u043du0432u0441u0430u0434 dr. u043du0432VpreS 1 (20 - 68) for the exit of product of not less than 26.7% of the total protein. 3 u0441.u043f.u0444 - lu 3, table 2).