白藜芦醇对高糖环境下视网膜血管内皮细胞增殖及HMGB1乙酰化的影响

摘要

?AIM :To study potential mechanism and effects of resveratrol on the proliferation of human retinal vascular endothelial cells in high glucose environment. ? METHODS: Human retinal vascular endothelial cells were cultured in low glucoseor high glucose environment. The MTT assay was used to detect the proliferation of each group to study the effects of resveratrol on the proliferation of human retinal vascular endothelial cells in high glucose environment.Detection of Sirtuin 1 ( SIRT1 ) expression and acetylated high mobility group box-1 protein 1 ( HMGB1 ) was performed by western-blot and coimmunoprecipitation. ? RESULTS: Resveratrol inhibited the proliferation of human retinal vascular endothelial cells in high glucose in a dose-dependent manner.High glucose decreased SIRT1 expression andincrease the degree of HMGB1 deacetylation, which can be reversed by resveratrol. ?CONCLUSION:Resveratrol may reverse proliferation of retinal vascular endothelial cells in high glucose through SIRT1-HMGB1 pathway.%目的：探讨白藜芦醇对高糖环境下视网膜血管内皮细胞增殖的影响，并对其机制进行探讨。　　方法：人视网膜血管内皮细胞培养于低糖或高糖环境，通过MTT法测定各组细胞增殖，研究白藜芦醇对高糖培养的视网膜血管内皮细胞增殖的影响。通过Western－blot及免疫共沉淀检测SIRT1表达及HMGB1的乙酰化。　　结果：白藜芦醇对高糖环境下的视网膜血管内皮细胞增殖具有显著抑制作用（P＜0．05），且随白藜芦醇剂量增加，抑制作用增强。高糖抑制SIRT1表达，提高HMGB1的乙酰化，白藜芦醇能逆转上述改变。　　结论：白藜芦醇可能通过SIRT1－HMGB1通路抑制高糖环境下的视网膜血管内皮细胞增殖。