白藜芦醇对高糖环境下视网膜血管内皮细胞线粒体DNA的影响及机制

摘要

目的:探讨白藜芦醇对高糖环境下视网膜血管内皮细胞线粒体DNA的影响及机制.方法:人视网膜血管内皮细胞培养于正常或高糖环境,提取细胞基因组DNA,Real-time PCR检测线粒体DNA拷贝数,研究白藜芦醇对高糖培养的视网膜血管内皮细胞线粒体DNA的影响.通过Western-blot及免疫共沉淀检测线粒体转录因子A(mitochondrial transcription factor A,TFAM)表达及PGC-1α乙酰化.结果:高糖对视网膜血管内皮细胞线粒体DNA的拷贝数有明显的抑制作用,白藜芦醇能够降低视网膜血管内皮细胞PGC-1α的乙酰化,提高TFAM的表达,增加线粒体DNA的拷贝数.结论:白藜芦醇可能通过激活PCG-1α-TFAM信号通路改善高糖环境下视网膜血管内皮细胞的线粒体功能.%AIM:To investigate effects of resveratrol on mitochondrial DNA copy in retinal vascular endothelial cells cultured under high glucose conditions and its mechanism.METHODS:Human retinal vascular endothelial cells were cultured in low glucose or high glucose medium,the genomic DNA was extracted and copy number of mitochondrial DNA was detected by real-time PCR.Effects of resveratrol on the mitochondrial DNA copy in retinal vascular endothelial cells cultured under high glucose medium were studied.The expression of mitochondrial transcription factor A (TFAM) and acetylated proliferator-activated receptor coactivator-1 alpha (PGC-1α) were analyzed by Western blot and coimmunoprecipitation.RESULTS:High glucose inhibited the copy number of mitochondrial DNA in retinal vascular endothelial cells.However,resveratrol decreased the level of acetylated PGC-1α in retinal vascular endothelial cells,increased the expression of TFAM and the copy number of mitochondrial DNA.CONCLUSION:Resveratrol may improve mitochondrial function of retinal vascular endothelial cells exposed to a high glucose environment via activation of the PCG-1α-TFAM signaling pathway.