ATP法测水中细菌数目效果评价及预警限值研究

摘要

This study uses ATP bioluminescence method and plate counting method to determine the total number of bacteria in single Escherichia coli,pseudomonas fluorescens,two kinds of mixed bacteria and the total number of bacteria in domestic hot water and drinking water,the effect of ATP method on the determination of the total bacteria number in water is evaluated and the alert thresholds of the bacteria number is also explored in domestic hot water and drinking water. In addition,it is discussed the factors that influence the relationship between ATP bioluminescence method and plate counting from three aspects,microbial species,nutrient and the medium used for plate counting. The results show that,ATP biofluorescence method can be used as a primary screening method,to evaluate whether domestic hot water and drinking water is qualified and when detecting the total bacteria number of domestic hot water and drinking water with Pi-102 fluorescence detector,the recommended alert thresholds should be 1 000 RLU and 1 400 RLU respectively.%通过采用ATP法和平板计数法测定单一的大肠杆菌、荧光假单胞菌、两种混合菌菌悬液以及生活热水和饮用水的细菌总数,对ATP法测定水体中细菌总数进行了效果评价和测定生活热水、饮用水中细菌数目预警限值的研究,并从水体中微生物的种类、营养物质、平板计数法所用培养基3个方面探讨了对ATP法和平板计数法间相关指数R2值的影响.结果表明,ATP生物荧光法可以作为评价生活热水和饮用水是否合格的一个初筛方法,用Pi-102型荧光检测仪检测生活热水和饮用水细菌数目时,建议参考的预警限值分别为1000 RLU和1400 RLU.