采用ATP生物发光法分析6株常见细菌ATP含量差异

摘要

以ATP生物发光法为基础,辅以国标平板计数法,分析和测定了大肠埃希氏菌、枯草芽孢杆菌、蜡状芽孢杆菌、乳酸链球菌、乳酸片球菌等6株常见细菌中ATP含量的差异.结果显示:当单位体积菌悬液的ATP荧光值相同时,6株细菌菌落总数无明显差异;当菌悬液的菌落总数相同时,6株细菌的单位体积菌悬液ATP含量无明显差异.研究结果同时说明ATP生物发光法能够定量测定常见区域中的细菌总数.%ATP bioluminescent method and plate count method were used to analyze and determine the ATP content of six common strains of bacteria,which were Escherichia coli,Bacillus subtilis,Bacillus cereus,Streptococcus lactis and Pediccoccus acidilactici.The results showed no obvious difference among aerobic plate counts of six bacterial strains with same fluorescent values,while there was little difference among the ATP contents of six strains with same aerobic plate counts.It interpreted that the total bacteria in common area could be quantitatively detect by ATP bioluminescent method.