富硒双歧杆菌缓解5-FU导致的化疗性肠粘膜炎的药效学研究

摘要

通过腹腔注射5-FU建立小鼠肠黏膜炎模型,探讨富硒长双歧杆菌(Selenium-enriched Bifidobacterium longum,Se-B.longum)能否改善5-FU所致的小鼠肠黏膜炎.将健康BALB/c小鼠随机分为对照组、5-FU组和Se-B.longum/5-FU组,分别灌胃生理盐水、生理盐水和Se-B.longum(Se 0.3 mg/kg BW,1×106 bacteria/只)6 d,然后5-FU组和Se-B.longum/5-FU组小鼠均腹腔注射5-FU(250 mg/kg),观察小鼠腹泻及死亡情况,5 d后处死小鼠,计算体重变化、脏器指数及考察肠道组织变化;将健康BALB/c小鼠随机分为对照组、5-FU组和Se-B.longum/5-FU组,分别灌胃生理盐水、生理盐水和Se-B.longum(Se 0.3 mg/kg BW,1×106 bacteria/只)6 d,然后5-FU组和Se-B.longum/5-FU组小鼠均腹腔注射5-FU(300 mg/kg),观察小鼠死亡情况,绘制生存曲线.Se-B.longum能缓解5-FU导致的正常小鼠的肠粘膜炎、降低小鼠死亡率.%In order to evaluate the effects of selenium-enriched Bifidobacterium longum (Se-B.longum) on 5-FU-induced intestinal mucositis in healthy mice,Se-B.longum (Se 0.3 mg/kg BW,1×106 bacteria/mice) was administered to mice for 6 days before administration of 5-FU (250 mg/kg,single dose).Body weight loss,liver index,spleen index,diarrhea and impaired small intestine induced by 5-FU was significantly alleviated by Se-B.longum (P<0.05).Se-B.longum (Se 0.3 mg/kg BW,1×106 bacteria/mice) significantly decreased mortality induced by 5-FU (300 mg/kg,single dose).As a result,Se-B.longum could relieve the intestinal mucositis and the mortality induced by 5-FU in normal mice.