A metabolic control breeding strategy and a method to improve the efficiency of mutants selection based on the analysis of the biosynthetic pathway of spinosad metabolism.Taking the strain Saccharo polyspora spinosa QYLZ 88912 as the original strain,a high-yield strain S.spinosa SG+St+Er+-088 of spinosad with muti-resistance was obtained after UV mutation.This strain had the resistance of sulfa guanidine,streptomycin and erythromycin.Its production of spinosad reached 1 132.60 mg/L under the level of shake flask fermentation through 7 days,which was 85.4％ higher than that of the original strain S.spinosa QYLZ 88912.The subculture experiments indicated that the hereditary characteristic of high productivity of S.spinosa SG+St+Er+-088 was relatively stable.%通过对刺糖多孢菌(Saccharopolyspora spinosa)多杀菌素代谢合成途径的分析,提出了一种代谢控制育种策略和提高诱变筛选效率的方法.以刺糖多孢菌QYLZ 88912菌株为出发菌株,选择不同照射时间分别对其进行紫外线照射处理,然后用含有不同抗性物质的摇瓶对诱变菌进行初筛,最终筛选出了1株高产多杀菌素的多抗性菌株SG+St+Er+-088.该菌株同时具有磺胺胍、链霉素和红霉素抗性,摇瓶发酵试验表明,发酵7d多杀菌素浓度可以达到1 132.60 mg/L,较出发菌株提高了85.4％,经传代试验证明此菌株高产遗传特性稳定.
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