苏州荸荠组培快繁技术研究

摘要

[Objective] The study aimed to establish the rapid propagation in vitro and regeneration system of Eleocharis dulcis. [Method] With terminal bud of Eleocharis dulcis as explants, MS basic medium was used as the media by adding different concentration of 6-BA and NAA, the induction and proliferation culture tests were carried out on Eleocharis dulcis so as to research the effect of hormone with different concentration on induction and proliferation culture of Eleocharis dulcis and screen the optimal medium for rapid propagation and regeneration system of Eleocharis dulcis. [Result] The concentration ratio of NAA and 6-BA was the main cause of explants proliferation. The optimum induction medium for Eleocharis dulcis was MS＋ 0.20 mg/L 6-BA＋ 0.02 mg/L NAA, on which the induction rate was 81.40%. The appropriate proliferation medium for Eleocharis dulcis was MS＋0.50 mg/L 6-BA＋0.10 mg/L NAA, on which the propagation coefficient reached 3.8. [Conclusion] This study provided the basis for improving the tissue culture propagation techniques of Eleocharis dulcis.%[目的]建立适宜的苏州荸荠无性繁殖体系。[方法]以苏州荸荠顶芽为外植体,以MS为基本培养基,通过添加不同浓度的6-BA和NAA,对荸荠进行丛生芽诱导和增殖培养,研究不同激素浓度对苏州荸荠诱导和增殖培养的影响,为苏州荸荠快繁筛选最佳培养基。[结果]培养基中NAA和6-BA浓度比是影响苏州荸荠外植体增殖的主要原因。芽诱导的最适培养基为MS＋0.20mg/L6-BA＋0.02mg/LNAA,诱导效率达81.40%,丛芽增殖的较优培养基为MS＋0.50mg/L6-BA＋0.10mg/LNAA,丛芽增殖系数达3.8以上。[结论]该研究为完善苏州荸荠组织培养快繁技术提供依据。