荸荠‘店头三王’高效组培快繁及驯化移栽技术

摘要

In order to establish a rapid propagation system of Eleocharis dulcis cv. Diantou Sanwang by tissue culture , the corm terminal buds of Diantou Sanwang was used as explants, and basic MS medium supplemented with 6-BA, NAA, AC and PVP at various concentrations was used. The results showed that the optimal induction medium was MS+6-BA 1.5 mg.L-1. For proliferation of the shoots, MS+6-BA 1.5 mg.L-1 + NAA 0. 01 mg-L"1 was the best medium, and the highest proliferation rate reached 11. 2. The optimal combination of rooting medium was modified as 1/2 MS + NAA 0.05 mg.L-1 +AC 1 000 mg-L"1 or MS + NAA 0. 05 mg-L"1 +AC 1 000 mg.L-1 on which 100% rooting rate was induced. We also found that it could be better in controlling browning when we added 400 mg .L -1PVP during subculture, and browning rate decreased from 89. 6% to 2. 0% , meanwhile, the multiplication coefficient increased from 11. 20 to 13. 85. In this paper, we also made a detailed study on the tissue culture acclimation and transplanting techniques, and the seedling survival rate was up to 100%.%以台州本地荸荠‘店头三王’球茎顶芽为外植体,以MS为基本培养基,添加不同浓度6-BA,NAA,AC,PVP等诱导剂,结果显示:适宜的诱导培养基为MS+BA 1.5 mg·L-1；适宜的增殖培养基为MS+ 6-BA1.5 mg·L-1 +NAA 0.01 mg·L-1,增殖系数为11.2,适宜的生根培养基为1/2MS +NAA 0.05 mg·L-1 +AC 1000 mg·L -或者MS+ NAA 0.05 mg·L -+ AC 1 000 mg·L -,生根率达100％.并且提出了荸荠继代培养中以添加400 mg·L-1PVP防治褐化现象效果较好,株褐化率从89.6％下降到2.0％,增殖系数从11.20提高到13.85.采用本项目荸荠组培苗的驯化、移栽技术,假植秧田移栽成活率高达100％.