Objective To develop a method for determination of paeoniflorin in xiao'er huadu powder san by HPLC. Methods Chromatography was carried out on a column of HiQ sil C18 W(4.6 mm×250 mm,5 μm) ,at 30 ℃.. The mobile phase was composed of acetonitrile-0.1% phosphoric acid(83 : 17). The flow rate was 1.0 mL·min-1 ,and the detention wavelength was set at 230 nm. Results The content of paeoniflorin showed a good linearity in the range of 0. 243-2. 430 μg and the average recovery was 100. 1% ,with RSD as 1.61% (n = 6). Conclusion The method is simple,rapid,accurate,reproducible and can be used for the quality control of the preparation.%目的 建立检测小儿化毒散中芍药苷含量的高效液相色谱法.方法 色谱柱HiQ sil C18W(4.6 mm×250 mm,5μm),柱温:30℃,流动相:乙腈-0.1%磷酸(83∶17),流速:1.0 mL·min-1,紫外检测波长:230 nm.结果 芍药苷含量在0.243 ~2.430 μg范围内有良好线性关系,平均加样回收率100.1%,RSD=1.61%(n=6).结论 该方法简便,快速,准确,重复性好,可用于该制剂质量控制.
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