拉莫三嗪对大鼠急性分离海马神经元钠电流的影响

摘要

Objective To investigate the effect of lamotrigine (LTG) on sodium channel in acutely isolated hippocampal neurons of rats. Methods The hippocampal neurons of rats at the age of 7-10 d were prepared by chain wire fungus protease E and blowing. The effects of LTG on voltage-dependent sodium channels were determined by using whole-cell patch clamp technique. Results The amplitude of voltage-dependent sodium currents were decreased (15. 35±3.01) % by LTG in a concentration-dependent manner at the dose of 100 μmol · L . Moreover, LTG shifted the activation curve of sodium currents by (3.8±0.9) mV toward depolarization and the steady state inactivation curve of these currents by (1.8±0.9) mV toward hyper-polarization. Conclusion LTG can block voltage-dependent sodium channels.%目的 探讨拉莫三嗪对酶机械联合急性分离的大鼠海马神经元细胞的钠通道的作用.方法 采用7～10 d大鼠,以链丝菌蛋白酶E加吸管吹打法制备海马神经元,应用全细胞膜片钳技术,观察拉莫三嗪对急性分离大鼠海马神经元电压依赖性钠通道的作用.结果 拉莫三嗪使电压依赖性钠电流幅值减小,100 μmol·L-1拉莫三嗪使钠电流幅值减少(15.35±3.01)％,这种作用具有浓度依赖性；使钠电流的激活曲线向去极化方向漂移(3.8±0.9) mV,稳态失活曲线超极化方向漂移(1.8±0.9)mV.结论 拉莫三嗪对电压依赖性钠离子通道有阻断作用.