超快速液相色谱法测定三七胶囊中5种皂苷含量

摘要

目的 建立测定三七胶囊中5种皂苷含量的超快速液相色谱法.方法 以Shimadzu C18(75 mm×2.0 mm,2.2 μm)为色谱柱,柱温25℃,乙腈-水梯度洗脱,流速0.3 mL· min-1,检测波长203 nm.结果 三七皂苷R1在9.38～351.62 ng(R2=1),人参皂苷Rg1在52.11～1 954.12 ng(R2=0.999 9),人参皂苷Re在6.85 ～ 152.17 ng(R2 =0.999 6),人参皂苷Rb1在44.99 ～1 687.25 ng(R2=1),人参皂苷Rd在9.96 ～221.27 ng(R2 =1)范围线性关系良好；加样回收率分别为96.6％(RSD=0.9％),97.1％(RSD=1.4％),101.0％(RSD=1.4％),97.9％(RSD=2.4％),98.2％(RSD=1.8％).结论 该方法简单,快速,适用于测定三七胶囊中五种皂苷含量.%Objective To establish an ultra fast liquid chromatography ( UFLC ) method for determining five saponins in sanqi capsule.Methods Chromatography was performed on an shimadzu C18 column (75 mm×2.0 mm, 2.2 μm) at 25℃.The gradient elution was acetonitrile-water at a flow rate of 0.3 mL·min-1.The detection wavelength was at 203 nm.Results The linear ranges for notoginsenoside R,,ginsenoside Rg,,ginsenoside Re, ginsenoside Rb, and ginsenoside Rd were 9.38-351.62 ng (R2 = 1), 52.11-1 954.12 ng (R2=0.999 9), 6.85-152.17 ng (R2=0.999 6), 44.99-1 687.25 ng (R2= 1),9.96-221.27 ng (R2= 1),respectively.The average recoveries were 96.6% for notoginsenoside R1( RSD = 0.9% ),97.1% for ginsenoside Rg1(RSD= 1.4%),101.0% for ginsenoside Re ( RSD= 1.4% ), 97.9% for ginsenoside Rb1 ( RSD = 2.4% ),98.2% for ginsenoside Rd (RSD= 1.8%), respectively (n= 6).Conclusion The method is simple, rapid and suitable for determination of five saponins in sanqi capsule.