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Liquid chromatography measurement method, liquid chromatography measurement instrument, and liquid chromatography measurement program storage medium

机译:液相色谱法测定方法,液相色谱法测定仪器以及液相色谱法测定程序存储介质

摘要

A liquid chromatography measurement method includes: switching between a first measurement mode using a liquid chromatography method in which hemoglobin A1c and a hemoglobin variant are measured in a measurement sample by sequentially delivering a first component-separating eluent, a second component-separating eluent and a wash eluent to an analytical column, and a second measurement mode using the liquid chromatography method in which the hemoglobin A1c is measured by sequentially delivering the first component-separating eluent and the wash eluent to the analytical column; delivering the wash eluent in the first measurement mode prior to an influence from the second component-separating eluent disappearing such that a first retention time of the hemoglobin A1c in the first measurement mode and a second retention time of the hemoglobin A1c in the second measurement mode are substantially the same as each other; and delivering the first component-separating eluent after the wash eluent.
机译:液相色谱法的测定方法包括:在液相色谱法的第一测定模式之间进行切换,在该第一测定模式中,通过依次输送第一成分分离洗脱液,第二成分分离洗脱液和第二色谱分离液来测定测定样品中的血红蛋白A1c和血红蛋白变异体。将洗提液洗至分析柱,并使用液相色谱法的第二测量模式,其中通过将第一成分分离洗提液和洗提液依次输送至分析柱来测定血红蛋白A1c。在第二组分分离洗脱液的影响消失之前,以第一测量模式输送洗涤洗脱液,使得在第一测量模式下的血红蛋白A1c的第一保留时间和在第二测量模式下的血红蛋白A1c的第二保留时间彼此基本相同;在洗涤洗脱液之后输送第一组分分离洗脱液。

著录项

  • 公开/公告号US10527594B2

    专利类型

  • 公开/公告日2020-01-07

    原文格式PDF

  • 申请/专利权人 ARKRAY INC.;

    申请/专利号US201715490320

  • 发明设计人 AKIRA SEZAKI;TAKESHI TAKAGI;

    申请日2017-04-18

  • 分类号G01N30/38;G01N30/86;G01N33/49;G01N30/02;

  • 国家 US

  • 入库时间 2022-08-21 11:19:13

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