葎草颗粒对慢性支气管炎模型小鼠的作用

摘要

目的：探讨葎草颗粒对实验性慢性支气管炎小鼠的作用及其作用机制。方法将小鼠随机分为正常对照组，右美沙芬组或氯化铵组，葎草颗粒大剂量、中剂量、小剂量组(分别灌胃葎草颗粒溶液3.00，1.50，0.75 g��kg－1)，观察葎草颗粒对浓氨水致咳小鼠影响和对小鼠气管酚红排泌量的影响。采用香烟加甲醛熏法复制慢性支气管炎小鼠模型，将60只小鼠按随机数字法分为6组：正常对照组，模型对照组，桂龙咳喘宁胶囊组，葎草颗粒大剂量、中剂量、小剂量组，每组10只。从造模第18天起，正常对照组和模型对照组小鼠灌胃0.9％氯化钠溶液，葎草颗粒大剂量、中剂量、小剂量组小鼠灌胃葎草颗粒，剂量分别为3.00，1.50，0.75 g��kg－1；桂龙咳喘宁胶囊组小鼠灌胃桂龙咳喘宁胶囊内容物，剂量为600 mg��kg－1，每天1次，共15 d。观察各组小鼠肺组织白细胞介素-8( IL-8)、肿瘤坏死因子-α( TNF-α)的含量。结果通过苏木精-伊红( HE)染色观察发现，葎草颗粒能明显减轻小鼠慢性支气管炎的损伤程度；正常对照组、葎草颗粒大剂量、中剂量、小剂量组2 min内分别咳嗽(17.50±5.38)，(11.90±4.46)，(12.60±3.47)，(9.50±3.24)次；酚红分泌量分别为(0.52±0.11)，(0.65±0.15)，(0.64±0.14)，(0.67±0.19)μg��mL－1；葎草颗粒能明显减少氨水导致的小鼠咳嗽次数，增加小鼠气管酚红排泄量。正常对照组，模型对照组及葎草颗粒大剂量组肺组织TNF-α的含量分别为(25.8±6.9)，(66.3±11.7)，(43.5±7.7) ng��mg-1，肺组织IL-8的含量分别为(27.1±9.1)，(48.2±11.4)，(36.5±8.2) ng��mg－1。模型对照组TNF-α、IL-8含量均较正常对照组明显升高( P<0.01)；与模型对照组比较，大剂量葎草颗粒可明显降低肺组织中TNF-α和IL-8的含量( P<0.01或P<0.05)。结论葎草颗粒对慢性支气管炎模型小鼠病理损伤有明显改善作用，并具有镇咳和祛痰作用，其作用机制可能与降低TNF-α、IL-8含量有关。%Objective To study the effects and the therapeutic mechanism of Humulus scandens particles ( HSP ) on the mice with chronic bronchitis ( CB ) . Methods Mice were divided into normal control group, dextromethorphan group or ammonium chloride group, the high-, middle-and low-dose of HSP groups ( These three groups received gavage administration of HSP at 3.00 g��kg-1, 1.50 g��kg-1 and 0.75 g��kg-1,respectively) randomly.The effects of HSP on ammonia-induced cough and trachea phenol red excretion in mice were observed.CB mouse model was established by inhaling smoke and formaldehyde.In total, 60 mice were divided into 6 groups by the method of random digits table: normal control group, model control group, Guilong kechuanning capsule group, the high-, middle-and low-dose HSP groups (n=10 each group).After modeling for 18 days, mice in normal control group and model control group were administered intragastrically with 0. 9% sodium chloride solution. Mice in the high-, middle-and low-dose HSP groups were daily treated with HSP at 3.00, 1.50 and 0.75 g��kg-1 ,respectively, by intragastric administration.Mice in Guilong kechuanning Capsule group were administered with Guilong kechuanning Capsule (600 mg��kg-1), once a day, for 15 days.The tumor necrosis factor alpha ( TNF-α) & interleukin-8 ( IL-8) contents in lung tissues of each group were determined. Results By hematoxylin-eosin (HE) staining, it was found that HSP alleviated the damage of CB in mice.The frequency of cough in 2 min in the normal control group, the high-, middle- and low-dose HSP groups were (17.50±5.38), (11.90±4.46), (12.60±3.47), (9.50±3.24), respectively, and the ecretion of phenol red were (0.52±0.11), (0.65±0.15), (0.64±0.14), (0.67±0.19) μg��mL-1, respectively.The content of TNF-α in lung tissues of the normal control group, model control group,the high dose HSP group was (25.8±6.9), (66.3±11.7), (43.5±7.7) ng��mg-1, respectively.The content of IL-8 in lung tissues of the normal control group, model control group, the high-dose HSP group were (27.1±9.1), (48.2±11.4) and (36.5±8.2) ng��mg-1, respectively.The contents of TNF-α and IL-8 in lung tissues of the model control group were significantly higher than those in normal control group (both P<0.01).Compared with the model control group, high-dose HSP could obviously decrease the contents of TNF-α and IL-8 in lung tissues (P<0.01 or P<0.05). Conclusion HSP can obviously alleviate the pathomorphological changes in mice with CB and has antitussive and expectorant effecs.The therapeutic mechanism of HSP for CB may be related to decreasing the contents of TNF-αand IL-8 in lung tissue.