茄子高频再生体系的建立与优化

摘要

In order to establish and optimize the high-frequency regeneration system of eggplant ,taking cotyle-donary petiole and hypocotyl explants of Hanongzaqie 2 and Hanongzaqie 3 as materials ,the shoot regeneration frequencies of two eggplant(Solanum melongena L)cultivars were examined to discuss the factors affecting re-generation rate .The results showed that the optimum shoot inducing mediums for cotyledons was MS 0+6-BA 1 mg·L-1 +KT 2 mg·L-1 ;the optimum shoot inducing mediums for hypocotyls was MS 0+ KT 2 mg·L-1 ,the best rooting medium for regeneration seedling was 1/2MS+IAA 0 .1 mg·L-1 .And the differentiation of hypo-cotyls was higher than that of cotyledons .%为建立并优化茄子高频再生体系，研究以茄子品种哈农杂茄2号和哈农杂茄3号为材料，利用不同的外植体和植物生长调节剂组合，探讨影响茄子再生频率的主要因素。结果表明：茄子最佳再生子叶最适分化培养基为MS 0＋6-BA 1 mg·L-1＋KT 2 mg·L-1；下胚轴最适分化培养基为MS 0＋ KT 2 mg·L-1，再生苗最佳生根培养基为1／2MS＋IAA 0．1 mg·L-1，且下胚轴的分化效率高于子叶。