目的 探讨K167、CyclinD1在食管鳞状细胞癌中的表达意义及与食管鳞癌发生发展之间的关系.方法 应用流式细胞术方法(flow cytometry,FCM)检测60例食管鳞状细胞癌及食管正常组织中K167、CyclinD1蛋白的表达情况,并检测食管癌组织中Ki67、CyclinD1蛋白表达与不同临床参数之间的关系.FCM方法检测食管癌组织中Ki67与CyclinD1蛋白表达的相关性.结果 流式细胞术方法检测到食管癌组织中Ki67、CyclinD1蛋白表达水平显著高于正常食管组织(P<0.05).食管癌组织中Ki67、CyclinD1蛋白表达水平与食管鳞癌细胞分化程度、有无淋巴结转移、癌浸润深度有关(P<0.05),与患者的性别及年龄无关(P>0.05).食管癌组织中Ki67与CyclinD1蛋白表达呈正相关性.结论 Ki67、CyclinD1蛋白在食管癌组织中的高表达可能参与了食管癌的发生与发展,其高表达使细胞具有旺盛增殖能力,促进肿瘤发生.联合检测Ki67、CyclinD1可作为食管癌诊断指标.%Objective To investigate the expression and significance of Ki67 and CyclinDl protein in esophageal squamous cell carcinoma, and to explore the correlation between the expressions of Ki67, CyclinDl protein and pathogenesis as well as progression of esophageal squamous cell carcinoma. Methods The levels of Ki67,CyclinDl protein expression in 60 cases of normal esophageal mucosa and esophageal carcinoma tissues were detected by flow cytometry. The correlation between the expression of Ki67 ,CyclinDl protein and clinicopathological characteristics of esophageal squamous cell carcinoma was analyzed. Results The expression levels of Ki67,CyclinDl protein in esophageal squamous cell carcinoma were significantly higher than those in normal esophageal tissues( P ＜ 0.05). The expression levels of Ki67, CyclinDl protein in esophageal cancer were closely correlated with the degrees of differentiation, metastasis of lymph node and the invasive depth of the tumor ( P ＜ 0.05 ).There was no significant correlation between Ki67, CyclinDl protein expression in esophageal cancer and the patients'gender,age ( P ＞0.05). There was a positive correlation between ki67 protein expression and CyclinD1 protein expression in esophageal cancer. Conclusion The high-expression of Ki67 and CyclinDl may be involved in the pathogenesis and advancement of esophageal squamous cell carcinoma, which may induce the high proliferation of cells, then result in the tumorigenesis. The combination detection of Ki67 and cyclinDl can be used as valuable diagnosis indexes for esophageal carcinoma.
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