[Abts ract] Objective To investigate the protective effect of alpha-lipoic acid ( ALA ) on oxidative stress in the podocytes of rats with diabetic nephropathy.Methods After the rat models with diabetic nephropathy were successfully established,the 10 rats were randomly selected as diabetic nephropathy group (group B), the other 10 rats were regarded as ALA intervention group (group C),at the sime time, 10 normal rats were served as control group (group A).The animals were sacrificed at th end of 13weeks ,and the renal tissues specimens were harvested .The expression levels of nephrin and podocin were detected by Western Blot and immunohistochemistry.The contents of malondialdehyde ( MDA) in renal tissues were measured by thiobarbituric acid (TAB) method and the activities of total superoxide dismutase (T-SOD), glutathione peroxidase ( GSH-PX) in renal tissues were detected by xanthine oxidase and dithio-2 nitrobenzoic acid method,respectively. Resu lts As compared with those in group A,the concentrations of MDA in group B were significantly increased, however, the acticities of T-SOD ,GSH-PX and the expression levels of nephrin, podocin were significantly decreased ( P <0.05).As compared with those in group B, the concentrations of MDA were obviously decereased, but the acticities of TS-OD ,GSH-PX and the expression levels of nephrin, podocin were significantly increased in group C ( P <0.05).Conclusion ALA can inhibit the apoptosis of podocyte, reduce the urine protein and delay the pathogenesis and development of diabetic nephropathy through antioxidative stress effects.%目的：探讨硫辛酸对糖尿病肾病（DN）大鼠足细胞氧化应激的保护作用。方法制备糖尿病肾病（ DN）大鼠模型，成模后随机选取10只作为 DN组（ B组），10只作为硫辛酸干预组（ C组），并设立正常对照组（A组）。实验共13周，于第13周末收集各组大鼠肾组织标本，分别应用硫代巴比妥酸（ TAB）法、黄嘌呤氧化酶法、二硫代二硝基苯甲酸法测定肾脏中MDA含量和T－SOD、GSH－PX活性，应用western blot、免疫组化测定nephrin、podocin的表达。结果 DN组大鼠肾组织中MDA含量较A组明显升高，T－SOD、GSH－PX活性及nephrin、podocin的表达较A组明显下降（ P ＜0．05），而硫辛酸C组大鼠肾组织中MDA含量较DN组明显下降，T－SOD、GSH－PX活性及nephrin、podocin的表达较DN组明显升高（ P ＜0．05）。结论硫辛酸可通过抗氧化应激作用抑制足细胞的凋亡，减少尿蛋白，延缓DN 的发生发展。
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