黄体酮对视神经损伤大鼠视网膜神经节细胞微管相关蛋白-1B的影响

摘要

Objective To observe the effects of progesterone on the expression of microtubule associated protein-1B ( MAP-1B) in retinal ganglion cells of rats with optic nerve injury in order to provide experimental evidence of progesterone’ s protective effects on optic nerve.Methods Sixty rats were randomly divided into 3 groups,with 20 rats in each group.The rats in control group were not treated,however,the optic nerve injury models in right eye of rats were established in injury group 1, with 0.9%sodium chloride solution intraperitoneal injection,and the animal models were also established in injury group 2, with progesterone intraperitoneal injection.On 3d,7d,14d and 28d after injury, the right eyeballs of rats were excised, and retinal tissues were taken out, the morphological changes of retina were observed by HE staining, and the survival numbers of retinal ganglion cell were calculated, furthermore,the expression levels of MAP-1B in retinal ganglion cell were detected by immunohistochemistry.Results After optic nerve injury, the retinal nerve fiber layer appeared obvious edema, and the numbers of retinal ganglion cells were quickly decreased in injury group 1,however, the retinal morphological changes were alleviated,retinal nerve fiber layer appeared slight edema and the numbers of retinal ganglion cells were decreased slowly after treatment with progesterone in injury group 2.There was a significant difference in the optical density value at different time points between injury group 1 and injury group 2 ( P <0.05).Conclusion Progesterone can relieve the damage of retinal ganglion cells at early period of optic nerve injury through increasing the expression levels of cytoskeleton protein-MAP-1b, thus,which has protective effects on optic nerve and retina.%目的：观察并探讨黄体酮对视神经损伤早期视网膜神经节细胞微管相关蛋白-1B的影响，以期为黄体酮在视神经保护方面的作用提供实验依据。方法60只大鼠随机分为3组，每组20只。正常对照组不做任何处理，损伤1组制作右眼视神经夹伤模型，给予0)．9％氯化钠溶液腹腔注射，损伤2组制作右眼视神经夹伤模型，给予黄体酮腹腔注射。分别于损伤后1、3、7、14、28 d将3组大鼠右眼球摘除，取视网膜组织， HE染色光学显微镜观察视网膜形态学变化，并计数视网膜神经节细胞存活数量，免疫组织化学染色观察视网膜组织中MAP-1B在视网膜神经节细胞的表达情况。结果视神经损伤后损伤1组视网膜神经纤维层明显水肿，视网膜神经节细胞数目迅速减少，经黄体酮治疗的损伤2组视网膜形态改变轻微，视网膜神经纤维层轻度水肿，视网膜神经节细胞数目减少缓慢。损伤2组各时间段视网膜MAP-1B平均光密度值较损伤1组高，差异有统计学意义（ P ＜0．05）。结论黄体酮可以通过增加细胞骨架蛋白MAP-1B减轻视神经损伤早期视网膜神经节细胞的损害，对视神经及视网膜有保护作用。