目的：研究α-硫辛酸(Alpha-lipoic acid，ALA)对实验性变态反应性脑脊髓炎(Experimental autoimmune encephalomyelitis，EAE)大鼠模型中脊髓组织IL-17表达的影响。方法健康雌性Wistar大鼠50只，随机分为正常对照组(NC组)，EAE 7 d组(EAE-7 d组)、EAE 6个月病程组(EAE-6 m组)、硫辛酸急性期组(ALA-7 d组)和硫辛酸6个月病程组(ALA-6 m组)。HE染色观察病理学改变，免疫组织化学染色观察IL-17的表达变化。结果 HE染色结果显示，在α-硫辛酸治疗急性期组和6个月病程组，均可减轻脊髓组织的炎性反应和髓鞘损伤。免疫细胞化学染色结果显示，EAE-7 d组大鼠IL-17蛋白的表达比正常对照组增加(P<0.01)；EAE-6 m组大鼠IL-17蛋白的表达比正常对照组增加(P<0.01)；α-硫辛酸处理后急性期组和6个月病程组IL-17蛋白的表达明显降低(P<0.01)。结论α-硫辛酸在EAE大鼠模型中的保护作用可能是通过抑制IL-17的表达来实现的。%Objective To explore the effects of alpha-lipoic acid (ALA) on IL-17 expression in rats with ex-perimental allergic encephalomyelitis (EAE). Methods Fifty female Wistar rats were divided randomly into five groups: the normal control group (NC group), EAE model group with 7 days (EAE-7 d group), EAE model group with 6 months (EAE-6 m group), ALA administrated EAE model group with 7 days (ALA treated EAE-7 d group), ALA administrated EAE group with 6 months (ALA treated EAE-6 m group). HE staining was used to detect the pathologic changes. Immunohistochemistry was used to detect the expression of IL-17. Results HE staining showed that, in alpha-lipoic acid treated groups (ALA treated EAE-7 d group, ALA treated EAE-6 m group), inflammatory reac-tion and myelin damage were all relieved. According to immunohistochemistry, the expression of IL-17 in EAE-7 d group and in EAE-6 m group was significantly increased compared to NC group (P<0.01). However, the expression of IL-17 in alpha-lipoic acid treated groups significantly decreased compared with EAE-7 d group and EAE-6 m group re-spectively (P<0.01). Conclusion Alpha-lipoic acid may exert its neuroprotective effect through ameliorating inflamma-tive reacts.
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