黑豆皮原花青素提取工艺的优化

摘要

In order to optimize extraction conditions, extract technology of procyanidins from black soybean seed coat was explored base on single factor test and orthogonal test. Taking ethanol as the extraction agent. The effects of four factors such as ethanol concentration, extraction time, solid-liquid ratio and extraction temperature were studied. The optimum conditions of extraction were obtained. The results showed that the best extraction conditions was 60% ethanol as solvent, extracting time 80 minutes, solid-liquid ratio of 1 ∶ 25 and extracting temperature 60℃. Under these conditions,extraction rate for the first time was 85. 51%, and combined extraction rate could reach up to 95. 2%.%为了优化黑豆皮中原花青素的提取工艺,以乙醇为提取剂,考察了乙醇浓度、提取时间、料液比及提取温度4个因素对黑豆皮原花青素提取率的影响,并通过正交试验对最佳提取工艺进行了筛选.结果表明:乙醇浓度60％、提取时间80 min、料液比1:25、提取温度60℃为最佳提取工艺条件,原花青素1次提取率可达85.51％,合并提取率可达95,2％.