茯苓皮中三萜成分的分离鉴定及其生物防治活性

摘要

For the further study on biocontrol activity of triterpenoids from the peel of P .cocos,nine triterpenoids were isolated from the peer of P .cocos by silica gel column chromatography in this paper. Their structures were identified as dehydroeburicoic acid (1 ),dehydroeburicoic acid monoacetate (2), dehydroeburiconic acid (3),3-epi-dehydrotumulosic acid (4),poricoic acid D (5),poricoic acid C (6),25-hydroxy-3-epidehydroetumulosic acid (7 ),16-deoxyporicoic acid B (8 ),3α,16α-dihydroxylanosta-7,9 11),24-trien-21-oic acid (9)on the basis of spectroscopic data analyses.Compounds 4-7 exhibited definite toxicities against Ralstonia solanacearum with the range of inhibition zone 3.00~5.90 mm and compounds 1-5 and 9 had the marginal toxicities against Panagrellus redivivus with the range of corrected mortality rate 15.6%~30.3%.%为进一步研究茯苓皮中三萜成分的生物防治活性，采用硅胶柱层析色谱方法从茯苓皮中分离三萜类化合物，并通过波谱分析进行鉴定。结果表明：分离得到的9种三萜类化合物，分别为去氢齿孔酸（1）、去氢齿孔酸乙酰酯（2）、去氢齿孔酮酸（3）、3-epi-去氢土莫酸（4）、茯苓酸 D（5）、茯苓酸 C（6）、25-羟基-3-epi-去氢土莫酸（7）、16-脱氧茯苓酸 B（8）、3α，16α-二羟基羊毛甾-7，9（11），24-三烯-21-羧基（9）。化合物4-7对烟草青枯病菌（Ralstonia solanacearum）的抑菌圈范围为3．00～5．90 mm，化合物（1～5）和（9）对杀线虫（Panagrellus redivivus ）活性的校正致死率在15．6％～30．3％。