高胆红素血症大鼠听力损伤与耳蜗核CaM活性的相关性研究

摘要

目的 研究高胆红素血症大鼠听力损伤的机制及CaM活性变化在此损伤中的作用.方法 60只新生7 d SD大鼠,随机分为对照组(C组)和实验组(M组),测试各组动物脑干听觉诱发电位(ABR),检测各组血清胆红素浓度和脑组织胆红素含量,测试各组大鼠耳蜗核CaM的免疫组化平均灰度值.结果 M组用药后ABR反应阈明显升高,Ⅰ、Ⅱ、Ⅲ波潜伏期明显延长;M组耳蜗核CaM免疫组化平均灰度值明显低于C组.结论 CaM活性升高在高胆红素血症听力损伤过程中发挥重要作用.%Objective To investigate the effects of hearing damage with hyperbilirubinemia and function approach of neuro-toxicity.Methods Sixty newborn seven days SD(Sprague Dawley) rats were divided into contral group(C) and experiment group(M)randomly. Auditory brainstem response(ABR)and concentration of bilirubin in blood and brain in every groups were examined respectively. CaM immunohistochemistry dye were done in cochlear nucleus slices.Result ABR relfecting threshold obviously increases of group M after using medicine,Ⅰ,Ⅱ,Ⅲ wave delitescence obvious extend. The average gray value of cochlear nucleus CaM in group M are obviously lower than group C.Conclusion CaM activation rising play an important role in hearing damage in hyperbilirubinemia.