CdTe／CdS量子点对木犀草素与人血清白蛋白结合力的影响

摘要

Biocompatible and high luminescence CdTe/CdS core -shell quantum dots ( QDs ) were synthesized in aqueous solution using glutathione ( GSH) as sulfur source and stabilizer.Further, luteolin was studied for the affinities for human serum albumin ( HSA) in the presence and absence of CdTe/CdS QDs, respectively.Results revealed that the fluorescence intensities of HSA decreased remarkably with increasing concentration of QDs.Luteolin resulted in obvious red-shift of the maximum emission of HSA from 341 nm to 347 nm in the presence of QDs and the extent of red -shift induced by luteolin was much bigger than that in the absence of QDs.The binding constants and the number of binding sites per HSA molecule ( n) both increased.Thus, the binding affinities of luteolin for HSA increased in the presence of CdTe/CdS QDs.%用还原型谷胱甘肽（ GSH）作硫源和稳定剂在水相中合成了生物相容性好、量子产率高的核壳型CdTe/CdS半导体量子点；同时模拟人体内生理环境，研究了CdTe/CdS量子点（ QDs）对木犀草素与人血清白蛋白（ HSA）相互作用的影响。结果表明， CdTe/CdS QDs可有规律地猝灭HSA荧光；在CdTe/CdS QDs存在条件下，木犀草素可使HSA荧光最大发射波长从341 nm红移到347 nm，红移程度明显大于无QDs存在时，同时木犀草素与HSA的表观结合常数和结合位点数均有增加，即CdTe/CdS QDs使木犀草素与HSA的结合力增强。