杜仲叶绿原酸提取物对胰脂肪酶活性抑制的研究

摘要

通过对紫外分光光度计的运用来建立一种快速高效，低成本的方法来研究杜仲叶中绿原酸提取物对胰脂肪酶活性的影响。该实验中并未选用大型仪器来研究，而是选用常见的紫外分光光度计，实验过程中本法能达到良好的线性关系，其R＝0.9991，能够达到一般实验要求。实验结果得出杜仲叶中绿原酸提取物抑制脂肪酶的IC50值为0.00126 mg／mL，介于奥利司他与标准品之间，且高于标准品。%Ultraviolet spectrophotometer was using to establish a rapid and efficient, low cost method to study the effect on pancreatic lipase activity by the extract of chlorogenic acid from eucommia leaves.The study chose common UV spectrophotometer instead of large instrument in the experiment, in this experiment the method can achieve good linear relationship as R=0.9991, to achieve the general experimental requirements.The experimental results showed that extracts of chlorogenic acid from eucommia leaves inhibited lipase as IC50 value 0.00126 mg/mL, the result was between orlistat and standard, and higher than the standard.