非小细胞肺癌CDH13及DAPK基因CpG岛甲基化的研究

摘要

Objective To explore CpG island methylation and clinical significance of CDH13 and DAPK genes in non-small cell lung carcinoma patients.Methods Methylation specific PCR was used to detect the CpG island methylation status of CDH13 and DAPK genes among 40 patients with non-small cell lung carcinoma(lung cancer group) and 30 patients with benign lung diseases(benign lung disease group)R.esults The incidences of CpG island methylation of CDH 13 and DAPK genes were 47.5%(19/40) and 40.0%(16/40) respectively in the lung cancer group,27.5%(11/40) and 30.0%(12/40) respectively in the para-carcinoma group, and 0 in the benign lung disease group.The incidences of CpG island methylation of CDH13 and DAPK genes were significantly higher in the lung cancer group and para-carcinoma group in contrast with those in the benign lung disease group(P<0.05),which showed no significant difference between lung cancer group and para-carcinoma group(P>0.05).There was no significant difference in the incidences of CpG island methylation of CDH13 and DAPK genes between lung squamous carcinoma tissues and lung adenocarcinoma tissues(P>0.05).Conclusion The CpG island methylation of CDH13and DAPK genes can be observed in the non-small cell lung cancer tissues, and it is associated with the development of non-small cell lung carcinoma.%目的：探讨非小细胞肺癌患者抑癌基因CDH13及DAPK基因CpG岛甲基化情况及其临床意义。方法采用甲基化特异性-PCR方法检测40例非小细胞肺癌患者（肺癌组）和30例肺良性病变患者（肺良性病变组）的抑癌基因CDH13及DAPK的CpG岛甲基化的情况。结果肺癌组织 CDH13、DAPK 基因 CpG 岛甲基化率分别为47．5％（19／40）、40．0％（16／40），癌旁组织分别为27．5％（11／40）、30．0％（12／40），肺良性病变组织均为0。癌组织、癌旁组织CDH13、DAPK的CpG岛甲基化率均明显高于良性病变组（P＜0．05），但癌组织与癌旁组织间比较，差异无统计学意义（P＞0．05）。鳞癌与腺癌组织CDH13、DAPK的CpG岛甲基化率比较，差异均无统计学意义（P＞0．05）。结论非小细胞肺癌组织存在CDH13、DAPK的CpG岛甲基化，其与非小细胞肺癌发生发展有着密切关系。