BACKGROUND DAPK1 in gene transcription as a diagnostic marker of squamous cell carcinoma of the lung, applying levels of gene transcription DAPK1 DIAGNOSTIC squamous cell carcinoma of the lung, a method of diagnosing squamous cell carcinoma of the lungs and the primer set for determining the level of gene transcription DAPK1

摘要

1. The marker for diagnosing squamous cell lung cancer, which is the level of gene transcription DAPK1, wherein a decreased level in human suspected cancerous tissue as compared to its level in healthy tissue is diagnostic of cancer squamous legkih.2. Application DAPK1 gene transcription level as a marker for diagnosing squamous cell lung cancer cheloveka.3. A method of diagnosing lung, comprising the steps cancer squamous: a) obtaining the original pair of samples from patient tissue, wherein one of the samples obtained from suspected diseased tissue cancer, and the second is derived from adjacent histologically normal (conventionally normal) tissue, b) separating and purifying RNA preparations from samples of the original pair, c) synthesizing single-stranded or double-stranded cDNA on the RNA template using oligonucleotide primers, d) normalization DAPK1 cDNA concentration by the control gene transcription level ii which is constant in normal and lung cancer; d) carrying out a quantitative or semi-quantitative amplification reaction DAPK1 gene fragment using the cDNA obtained in step c) as a template and a pair genospetsifichnyh oligonuklestidnyh primers, e) comparing the amount of amplified DNA DAPK1 fragment sample obtained from suspected cancer affected tissue, with the amount of the amplified DNA fragment to a sample obtained from normal tissue, wherein the amount of the amplified fragment Nam DAPK1 reflect the level of transcription of the gene, wherein a decrease DAPK1 gene transcription is diagnostic of cancer squamous