The invention provides a primer group for detecting CpG island methylation of p16 gene using methylation specific fluorescence technique. The primer group comprises a pair of oligonucleotide primers and a fluorescence-labeled probe. Said oligonucleotide primer pair has base sequence represented by SEQ ID NO.1 and SEQ ID NO.2. Said fluorescence-labeled probe has base sequence represented by SEQ ID NO.3 or SEQ NO.4.
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机译:本发明提供了一种利用甲基化特异性荧光技术检测p16基因的CpG岛甲基化的引物组。引物组包含一对寡核苷酸引物和荧光标记的探针。所述寡核苷酸引物对具有由SEQ ID NO.1和SEQ ID NO.2表示的碱基序列。所述荧光标记探针具有由SEQ ID NO.3或SEQ NO.4表示的碱基序列。
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