槐猪FTO基因遗传多态性及其与肉质性状相关性研究

摘要

以上杭槐猪为试验材料,对FTO基因5′上游区域SNP g.-167 T>G、第一内含子SNP c.46-139A>T和第三外显子同义突变SNP c.594C>G 遗传多态性及其与肌内脂肪含量、背膘厚度、眼肌面积等肉质性状的相关性进行研究,3个突变分别产生EcoRⅠ、XapⅠ和Bsh1236I 等3个限制性内切酶酶切位点.结果表明:被测猪群在3个位点均存在一定的遗传多态性,PIC值分别为0.276、0.343和0.235,各位点等位基因型频率均处于Hardy-Weinberg 平衡状态;g.-167 T>G位点,等位基因G为优势等位基因,其基因频率为0.791,然而,TT基因型肌内脂肪含量显著高于GG基因型;c.46-139A>T位点,等位基因T为优势等位基因,其基因频率为0.674,相对于AA基因型,TT基因型显著降低了背膘厚度;c.594C>G 位点,等位基因G为优势等位基因,其基因频率为0.837,各基因型个体之间在3个被测性状中差异均不显著.因此,FTO基因g.-167 T>G和c.46-139A>T可作为槐猪肉质性状选育的潜在分子遗传标记.%PCR-RFLPs with EcoRⅠ,XapⅠ and Bsh1236 I restriction enzymes were used to analyze genetic diversities of three SNPs at the 5′-upstream region(g.-167 T>G),the first intron(c.46-139A>T) and the third exon(c.594C>G) of the FTO gene,and potential associations between genotypes and three meat quality traits including intramuscular fat(IMF),backfat thickness(BFT) and loineye area(LEA) were investigated. The results showed that three SNPs presented certain degrees of diversities with the PIC value being 0.276,0.343 and 0.235,respectively;and the frequencies of observed genotypes conformed to Hardy Weinberg equilibrium (P>0.05);for g.-167 T>G SNP,G allele was dominant with the frequency being 0.790,whereas CC genotype was statistically raised IMF content;for c.46-139A>T SNP,T allele was dominant with the frequency being 0.674,and TT genotype was statistically decreased BFT comparing toAA genotype;for c.594C>G SNP,G allele was dominant with the frequency being 0.837,and the SNP were not statistically associated with tested traits. In summary,two SNPs of g.-167 T>G and c.46-139A>T could be potential molecular genetic markers for the meat quality traits in the population of Huai pigs.