大黄联合乌司他丁对百草枯中毒大鼠肺损伤保护作用

摘要

目的：观察大黄联合乌司他丁对急性百草枯中毒大鼠肺组织病理变化以及血浆中TNF-α、TGF-β1含量的干预作用。方法将72只SD大鼠随机分为对照组8只、染毒组32只、干预组32只。染毒组、干预组分别于第1天、3天、5天、7天分批处死存活大鼠，光镜下观察肺组织变化，并测定血浆TNF-α、TGF-β1的含量。结果染毒组大鼠肺组织出现明显的出血、炎症细胞浸润、肺实变等变化；干预组较染毒组病理损伤减轻；染毒组、干预组TNF-α、TGF-β1水平在染毒后1天开始升高，与对照组相比，差异有统计学意义(P<0.01)，在相同时间点干预组血浆TGF-β1、TNF-α表达低于染毒组，差异均有统计学意义(P<0.01)。结论大黄联合乌司他丁可通过降低百草枯中毒大鼠血浆TNF-α、TGF-β1水平，发挥对百草枯中毒大鼠肺组织的保护作用。%Objective To evaluate the intervention effect of Rhubarb combine with Ulinastatin on pathological changes of lung tissue and the plasma levels of TNF -α and TGF β1 content on paraquat poisoning induced lung injury in rats. Methods 72 SD rats were randomly divided into control group (n=8), phosgene group(n=32), intervention group (n=32). The rats in phosgene group and the intervention group were sacrificed at first days, 3 days, 5 days and 7 days. The changes of lung tissue were observed under light microscope, and the determination of the content of plasma TNF-α, TGF-β1 . Results Lung tissue of phosgene group showed significant hemorrhage, inflammatory cell infiltration, pulmonary consolidation and other changes;The lung tissue of intervention group was better than the phosgene group;The level of TNF-α, TGF-β1 was increased on the first day after paraquat intoxication in phosgene group and intervention group, compared with control group, the result had statistical difference(P<0. 01); The plasma TGF-β1 and TNF-α of intervention group was lower than that of phosgene groups at the same time point, the differences were statistically significant(P<0. 01). Conclusions Rhubarb and Ulinastatin can reduce lung injury by decreasing the expression of TNF-αand TGF-β1 on paraquat poisoning induced lung injury in rats.