陕产铁棒锤不同生长期不同器官中3种酯型生物碱的含量测定

摘要

目的：对陕产铁棒锤不同生长期不同器官中乌头碱、次乌头碱及中乌头碱进行含量测定,探讨化学成分的动态变化,为资源开发及合理使用提供参考。方法色谱柱：Diamonsil C18(250 mm×4.6 mm,5μm)；流动相：A：乙腈-四氢呋喃(25：15),B：0.1 mol/L醋酸铵溶液(每1000 mL加冰乙酸0.5 mL)；流速：1.0 mL/min；梯度洗脱：检测波长为235 nm；温度25℃。结果铁棒锤不同生长期、不同器官中酯型生物碱含量均较高,不同器官中酯型生物碱的含量差异较大；块根在果期酯型生物碱的含量最高,茎在花前期酯型生物碱的含量最高,叶在花期酯型生物碱的含量最高；乌头碱在植物体内的含量呈现先升后降的变化趋势,花前期乌头碱的含量最高；次乌头碱在植物体内的含量呈现S变化趋势,苗期和花期的含量最高；中乌头碱在植物体内的含量呈现先升后降的变化趋势,花前期中乌头碱含量最高。结论铁棒锤不同生长期、不同器官酯型生物碱的含量远大于川乌的限量标准,且资源优质,具有较高的药用价值,本研究为陕产铁棒锤合理使用、资源开发及质量评价提供理论依据。%Objective To study the dynamic changes of chemical compositions in different organs of A. pendulum Busch from Shaanxi at different growing periods, and to provide a theoretical basis for the rational use and resource development, the method of HPLC was applied to analyze the contents of aconitine, hypaconitine and mesaconitine in different organs. Methods The assay was performed on Diamonsil C18(250 mm×4. 6 mm,5 μm) column at the temperature of 25℃ by gradient elution, mobile phase was made up of A and B at a flow of 1. 0 mL/min, and the A mobile phase consisted of acetonitrile and tetrahydrofuran(25:15), and the B mobile phase consisted of 0. 1 mol/L ammonium acetate solution (1000 mL solution mixed 0. 5mL glacial acetic acid), the eluent was monitored by UV detector at 235 nm. Results The content of three diester diterpenoid alkaloids were found to be much different and higher in different organs of A. pendulum Busch at different growing period; content of diester diterpenoid alkaloid were the highest in root during fruit stage;the highest contant of diester diterpenoid alkaloid in stem were at flower stage;the highest contant of diester diterpenoid alkaloid in leaf were at flower stage;The content of aconitine increased at first and then decreased at different growing period in phant and had the highest at prophase of flower stage;the content of hypaconitine showed changes in curve in plants and was the highest at seedling and flowering stage;the content of mesacontine increased at first and then decreased at different growing period in phant and the highest was at prophase of flower stage. Conclusion The medicinal value of A. pendulum Busch was higher in different organs at different growing period, the content of three diester diterpenoid alkaloids are much larger than that of aconiti Radix, this study provided a theoretical basis for the rational use, resource exploiting and quality evaluating of A. pendulum Busch produced in Shaanxi.