MDRV和H9AIV共感染对B淋巴细胞增殖功能的影响

摘要

To reveal the immunosuppression mechanisms, eight-day-old Muscovy ducklings, were challenged with Muscovy duck reovirus (MDRV) and avian influenza virus (H9AIV) individually and simultaneously. The changes of proliferation of B lymphocytes in the blood, spleens and bursa were determined by MTT method 7 days, 14 days and 21 days after the Muscovy ducklings were challenged. The results show that B lymphocyte stimulation indexes in the blood, bursa and spleen of the challenged group were significantly lower than that in control group, and B lymphocytes stimulation index in co-infection group was the lowest. So B lymphocytes proliferation of peripheral blood and humoral immune organs (bursas, spleens) in Muscovy ducklings was inhibited by both MDRV and H9AIV, and co-infection with MDRV and H9AIV could aggravate this immunosuppression.%以番鸭呼肠孤病毒(MDRV)或/和H9亚型水禽流感病毒(AIV)人工感染8日龄健康雏番鸭,应用MTT法测定感染后7、14和21 d番鸭血液、脾脏和法氏囊中的B淋巴细胞增殖功能的变化动态,探讨MDRV或/和H9亚型AIV对B淋巴细胞增殖功能的影响.结果显示8日龄番鸭人工感染后,MDRV感染、AIV感染和共感染组番鸭血液、法氏囊和脾脏的B淋巴细胞刺激指数均明显低于健康对照组,而且共感染组番鸭B淋巴细胞刺激指数低于MDRV感染和AIV感染组,表明MDRV和H9 AIV病毒感染均能抑制番鸭外周血液及体液免疫器官(法氏囊、脾脏)中B淋巴细胞增殖功能,MDRV与H9 AIV共感染加重B细胞免疫抑制程度.