摘要：
To elucidate the effect of Muscovy duck reovirus (MDRV) infection on the intestinal mucosal immunity in Muscovy ducklings,a total of 100 one-day-old MDRV antigen-antibody-negative Muscovy ducklings were randomly divided into two groups:normal control group (NCG) and cohabitation infection group (CIG).CIG was cohabitation infected with twenty artificial infection MDRV Muscovy ducklings basing on the previously established MDRV natural infection model.Duodenum,jejunum and ileum samples from NCG and CIG were collected at 1,3,6,10,15 and 21 days post-cohabitation-infection (dpi),respectively,and tissue sections were prepared for observation of morphological structure (villus height,villus width,villus height/crypt depth ratio,wall thickness and villus surface area) and determination of intraepithelial lymphocytes (IELs),goblet cells and mast cells counts.Results showed that MDRV infection caused obvious morphological changes of small intestinal mucosa in Muscovy ducklings from CIG,including destruction of villous structure,shedding of villous epithelial cells as well as increasing infiltration of intestinal submucosal inflammatory cells.Compared with NCG,villus height,villus width,villus density,V/C values,surface area of villus,thickness of intestinal wall,as well as numbers of intraepithelial lymphocytes,goblet cells and mast cells in Muscovy ducklings from CIG were remarkably decreased.And extremely significant differences (p＜0.01) of above indices were obtained from 6 dpi to 21 dpi.The results indicated that MDRV infection led to dysfunction of intestinal digestion and absorption and mucosal immunosuppression in sick Muscovy ducklings.The findings further enrich the pathogenesis of MDRV infection.%为探究番鸭呼肠孤病毒(MDRV)感染对雏番鸭肠黏膜免疫的影响,本研究将100只1日龄MDRV抗原抗体阴性雏番鸭随机分为二组:空白对照组(NCG)和MDRV同居感染组(CIG),利用人工感染发病的20只番鸭对GIC组番鸭进行同居感染.NCG和CIG组番鸭分别于后者同居感染1d、3d、6d、10d、15d、21d后采集其十二指肠、空肠、回肠样品进行肠道形态结构(绒毛高度、宽度、V/C值、肠壁厚度、绒毛表面积)的观察及免疫相关细胞(上皮内淋巴细胞、杯状细胞、肥大细胞)的检测.结果显示:与NCG相比,CIG番鸭肠道绒毛高度下降、宽度变窄,排列稀疏,V/C值降低,绒毛表面积下降,肠壁厚度变薄,上皮内淋巴细胞、杯状细胞、肥大细胞数量下降,尤其是同居感染6d以后其各项检查指标均与NCG差异极显著(p＜0.01).表明MDRV可导致肠道消化吸收功能障碍和黏膜免疫抑制.该研究结果进一步充实MDRV感染的致病机制.
