亚硒酸钠对海洛因吸食者 T淋巴细胞增殖及其线粒体功能的影响

摘要

Objective To study whether or not there is protective effect of sodium selenite on T lymphocytes of heroin addicts , and if it correlates with mitochondrial uncoupling related indicators .Methods T lymphocytes extracted from peripheral blood of people who abuse heroin in different periods .Cell proliferation was detected by MTT , the levels of ROS ,△Ψm were detected by fluorescence spectrophotometer , MPTP was measured by ultraviolet spectrophotometer .Results With the increased duration of heroin-abuse, T lymphocyte proliferation activity decreased significantly ( P<0.05); T lymphocyte mitochondrial MPTP increased , △Ψm decreased, ROS increased(P<0.05).Sodium selenite can reverse these changes ,and there is a dose-effect relationship(P<0.05).Conclusions Oxidative stress caused by prolonged heroin abuse has an adverse effect on the function and proliferation of T lymphocytes via the mi-tochondrial pathway .Sodium selenite has a protective effect on T lymphocytes by its antioxidant effect , which reduces the level of ROS to reverse the above process .%目的：探讨亚硒酸钠对海洛因吸食者T淋巴细胞的保护作用及其与线粒体解偶联相关指标的关系。方法使用不同海洛因吸食年限人员外周血，提取T淋巴细胞；四甲基偶氮唑蓝（ methyl thiazolyl tetrazolium ，MTT）法检测细胞生长情况，荧光分光光度法检测细胞内活性氧（ reactive oxygen species ，ROS）、细胞线粒体膜电位（ mitochondrial membrane potential ，△Ψm），用紫外分光光度计测线粒体通透性转运孔（mitochondrial permeability transition pore ，MPTP）。结果随着吸食海洛因的时间增加，T淋巴细胞的增殖活性下降，差异有统计学意义（ P＜0．05）；随着海洛因吸食年限的增加，T淋巴细胞线粒体MPTP增加、△Ψm下降、ROS升高（P＜0．05）；亚硒酸钠可逆转上述改变，且存在量效关系（P＜0．05）。结论长时间吸食海洛因所造成的氧化应激状态，通过线粒体途径对T淋巴细胞的功能和增殖情况有不利影响；亚硒酸钠可以通过其抗氧化作用，降低ROS水平来逆转上述过程，对T淋巴细胞具有保护作用。