香蕉枯萎病菌和芦笋茎枯病菌的拮抗放线菌筛选与鉴定

摘要

从江西井冈山地区采集的土壤样品中分离获得76株放线菌，通过对峙培养法筛选出菌株S15对香蕉枯萎病菌 Fusarium oxysporum、芦笋茎枯病菌 Phomopsis asparagi 有强抑制作用，该菌培养滤液对稻瘟病菌Magnaporthe oryzae、番茄灰霉病菌 Botrytis cinerea、大豆炭疽病菌 Colletotrichum truncatum、水稻纹枯病菌Rhizoctonia solani、柑橘炭疽病菌Colletotrichum gloeosporioides、香蕉黑星病菌Cladosporium cucumerinum、番茄早疫病菌 A lternaria solani、黄瓜炭疽病菌 Colletotrichum orbiculare、玉米大斑病菌 Setosphaeria turcica等多种重要的植物病原真菌也均有较强的抑制作用，拮抗谱较宽。根据其形态特征、培养特性、生理生化特性和16S rDNA序列同源性分析，S15被鉴定为链霉菌属波卓链霉菌 Streptomyces bottropensis。%Seventy-six strains of actinomycete were isolated from the soil in Jinggangshan area in Jiangxi province . S15 was found to be strongly antagonistic to Fusarium oxysporum and Phomopsis asparagi .The inhibition effect of S15fermentationfiltratewassignificantagainst Magnaportheoryzae,Botrytiscinerea,Colletotrichumtruncatum, Rhizoctonia solani , Colletotrichum gloeosporioides , Cladosporium cucumerinum , A lternaria solani , Colletotrichum orbiculare and Setosphaeria turcica . According to its morphology , cultural characteristics , physiological and biochemical characteristics and 16S rDNA sequence ,S15 were identified to be Streptomyces bottropensis .