液态培养红曲霉产洛伐他汀开闭环组分的研究

摘要

对红曲霉产开环莫纳可林 K 及闭环洛伐他丁的液态发酵工艺进行研究，探讨不同培养基成分和发酵条件对产洛伐他丁开闭环组分的影响。结果表明，最佳的变温培养方法是先30℃培养2 d 后再26℃培养7 d ，产生的开环莫纳可林 K 的比例可达87％，比26℃恒温培养的提高了2.63倍。当培养基中添加8％木糖、3％蛋白胨、0.2％ NaNO3、0.2％ KH2 PO4、0.35％ MgSO4，在接种量6％，摇床转速160 r ・ min -1，采用变温培养方法，得到的洛伐他汀类物质的产量最高，且开环莫纳可林 K 含量比基本培养基的提高了18倍。%The effects of medium compositions and culture conditions in Monascus liquid culture were studied on the production and the ratio of Monacolin K and Lovastatin . The optimal temperature-shift cultivation was 2 days culture at 30 ℃ followed with 7 days at 26 ℃ to increase the production of acid form Monacolin K .By this culture conditions ,the proportion of acid form Monacolin K in total Monascus products reached to 87% and it was 2.63 times of the ratio produced with constant temperature cultivation at 26 ℃ .Comparing with basic culture medium , additional 8% xylose ,3% peptone ,0.2% NaNO3 ,0.2% KH2 PO4 and 0.35% MgSO4 added into the basic medium was the optimal composition that produced the highest amount of Lovastatin compounds with the culture conditions in 6% inoculums size ,160r min - 1 rotate speed at the optimal temperature-shift cultivation .The ratio of acid form Monacolin K was 18 times of it produced by the basic medium .