毛竹内生细菌种群多样性分析

摘要

采用稀释平板法从福建武夷山毛竹根、鞭、杆、叶部组织中分离到27株内生细菌，利用细菌菌落表征性状和16S rDNA 序列对这些菌株进行了多样性分析。结果表明，分离出27株内生细菌分属于14属18种，其中以节细菌属 Arthrobacter、芽孢杆菌属 Bacillus 、苍白杆菌属 Ochrobactrum 和葡萄球菌属 Staphylococcus 为主要菌群。%Twenty-seven endophytic bacteria were isolated from the roots,rhizome,stems and leaves of moso bamboo by means of grinding separation.A preliminary determination on the diversity of the strains was made based on their phenotypic characteristics as well as the molecular classification using partial 16S rDNA sequences.The results showed that the isolated bacteria could be classified into 14 genera and 18 species,with Arthrobacter, Bacillus ,Ochrobactrum and Staphylococcus being the most predominant.