番鸭呼肠孤病毒弱毒疫苗在番鸭体内中和抗体消长规律的研究

摘要

To determine antibody response in the serum of muscovy ducks immunized with muscovy duck reovirusis (MDRV) alive vaccine ,the fluctuation of antibody titers was detected in the serum by fixed virus-serum dilutions method .The results showed that some muscovy ducks turned positive on antibody against MDRV after vaccination in 28th day ,while all of them appeared to be MDRV-antibody positive within 35 days ;the peak titer of the antibody showed at the 6th month after vaccination ,and the period of effective immunization could last over 18 months .The results told that there was a long delay between MDRV vaccination and antibody origin ,while the duration of antibody production was relatively long ,which provided scientific basis for immune procedure development .%为明确番鸭呼肠孤病毒弱毒疫苗在番鸭体内中和抗体消长规律，采用固定病毒稀释血清法，测定了经弱毒疫苗免疫后的雏番鸭不同时间其血清中和抗体效价。结果显示：疫苗注射1日龄雏番鸭，免疫后28 d部分鸭血清中出现抗体，35 d全部鸭血清中出现抗体；6个月抗体效价达高峰，有效免疫期在18个月以上。表明番鸭呼肠孤病毒弱毒疫苗诱导的中和抗体产生期较晚，但持续期长。