Purpose To detect the prevention of EGF and GLN on the side effect of TPN. Methods We randomized SD rats into four groups:group A (control),B(STPN),C(TPN+GLN),D(TPN+GLN+EGF).All rats were maintained on their respective diets for 8 days,at which time they were killed and the proximal jejunum was incised for biopsy,the MLN and celiac vein blood were collected for bacterial culture(aerobic culture). Results Group B rat mucosal cell atrophied,DNA and RNA content and plasma cell decreased,the positive rat of MLN bacterial culture was 62.5%.Atrophy in group C alleviated and bacterial translocation rate declined(37.5%).Group D was similar with group A. Conclusions GLN combined with EGF is more effective in protecting gut barrier function and preventing bacterial translocation than GLN alone.%目的探讨表皮生长因子(epidermal growth factor,EGF)、谷氨酰胺(glutamine,GLN)对全胃肠外营养(total parenteral nutrition,TPN)并发症的防治作用及机制。方法将大白鼠分为4组：A(正常喂养组)、B(标准胃肠外营养STPN)、C(TPN+GLN)、D(TPN+EGF+GLN)，采用大白鼠TPN模型，一周后取大鼠近端空肠组织切片行光镜和电镜观察，结合图像分析；无菌采取肠系膜淋巴结(mesenter lymphonodus,MLN)并称重和抽取腹腔静脉血作细菌培养(需氧培养)。结果 B组肠黏膜萎缩，MLN细菌培养阳性率62.5%。C组较B组肠黏膜萎缩减轻，黏膜DNA、RNA含量和浆细胞数目增加，MLN细菌培养阳性率37.5%，肠细菌移位率下降。而D组上述各指数与正常鼠A组无显著差异。4组动物血培养均为阴性。结论 EGF和GLN联合应用能有效地保护肠屏障功能和防止肠细菌移位
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