盐酸诺氟沙星在奥尼罗非鱼体内的生物利用度研究

摘要

The hybrid tilapia (Oreochromis aureus x 0. Niloticus) were treated with norfloxacin hydrochloride via intravenous injection and oral administration in medicated feed with a single dosage of 10 mg/kg body weight at water temperature of (25 ± 1 )℃ , and the norfloxacin hydrochloride was determined with high performance liquid ehromatography and analyzed by 3P97 practical phannacokinetics software. The pharmacokinetics and bioavailability of norfloxacin hydrochloride in hybrid tilapia were investigated. The results showed that the data of pharmacokinetics for intravenous injection conformed to a three-compartment model. The pharmacokinetic equation was: C1 = 1.441e-0.04t +5.561e-0.146t + 32. 68e-4.447t, and the main parameters were t1/2a=4. 759 h, ht1/2β = 17. 244 h, AUC =81. 342 μg · H/mL. The data of phannacokinetics for oral administration in medicated feed conformed to a two-compartment model. The pharmacokinetic equation was: Ct = 0. 219e-0.102(t-0.473) + 0.034e-0.018(t-0.473) -0.253e-6.977(t-0.473), and the main parameters were t1/2kg = 0.099 h, t1/2α = 6. 817 h, t1/2β = 38.411 h, Tmax = 0. 567 h, Cmax = 0.236 μg/mL, AUC =4.001 μg· H/mL. The bioavailability of oral administration in medicated feed was 4.99%. These results confirmed that the bioavailability of norfloxacin hydrochloride in hybrid tilapia was low under the oral administration of medicated feed. Therefore, the medicine was just suitable forthe prevention and treatmentof intestinal bacterial diseases.%采用高效液相色谱法分别测定静脉注射和混饲口灌奥尼罗非鱼(Oreochromis aureus×O.niloticus)盐酸诺氟沙星后鱼体血液中该药含量变化,用3P97药动学软件分析药动学参数,计算生物利用度.结果表明,在(25±1)℃水温下,以10 mg/kg剂量对奥尼罗非鱼静脉注射和混饲口灌盐酸诺氟沙星,静脉注射药时数据符合无吸收三室开放性模型,动力学方程为:Ct=1.441e-0.04t +5.561e-0.146t+ 32.68e-4.447t,主要动力学参数为:t1/2α=4.759 h、t1/2β=17.244 h、AUC =81.342 μg· h/mL;混饲口灌药时数据符合一级吸收具时滞的二室开放性模型,动力学方程为:Ct=0.219e-0.102(t-0.473)+0.034e-0.118(1-0.473)-0.253e-6.977(t-0.473),主要动力学参数为:t1/2ka=0.099 h、t1/2α =6.817 h、t1/2β=38.411 h、Tmax =0.567 h、Cmax==0.236 μg/mL、AUC =4.001 μg·h/mL.混饲口灌的生物利用度为4.99%,表明混饲给药下盐酸诺氟沙星在奥尼罗非鱼体内的生物利用度很低,仅适合肠道细菌性疾病防治.