本文采用正交实验的方法对不同提取条件下莲子心水提取液和乙醇提取液清除O2-·和·OH的能力进行了研究.结果表明:无论是莲子心的水提液还是莲子心的醇提液都表现出了良好的清除超氧阴离子和羟自由基的能力.相比而言,莲子心水提取液清除超氧阴离子的效果比清除羟自由基的效果好,而莲子心乙醇提取液清除羟自由基的效果比清除超氧阴离子的效果好.以综合抗氧化能力为指标,莲子心水提取液中抗氧化的成分提取的最佳条件为料液比为1:12,在90℃时提取0.5h;莲子心醇提取液中抗氧化的成分提取的最佳条件为料液比为1:14,在60℃时提取6h.%In this paper, the scavenging effects of lotus germ extraction on superoxide anion and hydroxyl free radical were studied with orthogonal test. The results showed that whether lotus germ water-extraction or ethanol-extraction having scavenging activities on superoxide anion and hydroxyl radical. Lotus germ water-extraction having higher scavenging effects on superoxide anion than on hydroxyl radical, but lotus germ ethanol-extraction having higher scavenging effects on hydroxyl radical than on superoxide anion. The final affirmed best conditons for water-extraction were extraction temperature 90℃, the ratio of sample/solvent (water) 1:12, extraction time 0.5h. The final affirmed best conditions for ethanol-extraction were extraction time 6h,extraction temperature 60℃, and the ratio of sample/solvent (water) 1:14.
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