党红饮体外保护线粒体功能评价

摘要

Objective： To explore the health benefits of Codonopsis pilosula/Vaccinium vitis-idaea drink（CVD） through evaluating its reactive oxygen species（ROS） scavenging capacity and protective effect on mitochondrial damage.Methods： The functional factors of CVD total flavonoids and Codonopsis polysaccharide were determined by AlC13 colorimetry and sulfuric acid-phenol method respectively.Lipid peroxidation in liver and brain mitochondria was induced by Fe2＋-vitamin C in vitro.TBA colorimetry was used to measure the content of thiobarbituric acid reactive substance（TBARS）.The scavenging activities of CVD against superoxide anion free radicals（O2-·） generated in reduced nicotinamide adenine dinucleotide（NADH）-N-methylphenazonium methyl sulfate（PMS） system and hydroxyl free radicals（·OH） generated in hydrogen peroxide（H2O2）-Fe2＋ system were measured by NBT reduction and Fenton reaction colorimetry,respectively.The Na2S2O3 titration method was used to measure the H2O2 scavenging activity of CVD.Permeability transition（PT） of liver mitochondria in rats was induced by Ca2＋ in vitro and measured spectrophotometrically.Results： The contents of total flavonoids and Codonopsis polysaccharide in CVD were（95.2 ± 6.3） μg/mL and（7.5 ± 0.4） mg/mL,respectively.Mitochondria incubated at a given temperature with Fe2＋-vitamin C system couldresult in an obvious increase of TBARS.CVD could inhibit TBARS production and significantly scavenge O2-·,·OH and H2O2 in a dose-dependent manner within a certain concentration range.CVD could protect mitochondria from PT induced by Ca2＋.Conclusion： The protective effect of CVD on mitochondrial damage can be achieved by antioxidation and scavenging ROS.Therefore,CVD has a heath-promoting effect.%目的：探索党红饮（CVD）的健康益处,研究其清除活性氧自由基特性并对线粒体损伤有保护作用。方法：分别用AlCl3比色法和酸苯酚法测定CVD的功能因子总黄酮及党参多糖含量。以Fe2＋-VC诱发肝线粒体脂质过氧化,采用TBA显色法测定硫代巴比妥酸反应物质（TBARS）含量;以还原型辅酶I-吩嗪硫酸甲酯（NADH-PMS）为超氧阴离子自由基（O-2.）生成系统,过氧化氢（H2O2）-Fe2＋体系为羟自由基（.OH）生成系统,分别用氮蓝四唑（NBT）还原法和Fenton反应显色法测定CVD清除O-2.及.OH的能力;用Na2S2O3滴定法测定CVD清除过氧化氢的能力;用Ca2＋诱导肝线粒体通透性转换（PT）,用分光光度法测线粒体的PT程度。结果：CVD总黄酮含量为（95.2±6.3）μg/mL,党参多糖含量为（7.5±0.4）mg/mL。线粒体与Fe2＋-VC体系共同温育,可使TBARS生成量显著增加,而加入CVD可明显抑制TBARS生成;CVD能在一定质量浓度范围内明显清除超氧O-2.、.OH和过氧化氢,并均呈剂量-效应关系;另外,Ca2＋引起的线粒体PT可通过加入CVD而一定程度抑制其PT的发生。结论：CVD能通过抗氧化及清除活性氧来保护线粒体免受损伤,具有保护机体的功效。