盐生海芦笋来源真菌Penicillium stoloniferum发酵产物抗肿瘤活性成分研究

摘要

Eight anti-tumor monomer compounds were isolated from the fermented broth and mycelia of the fungus Penicillium stoloniferum derived from Salicornia herbacea by bioassay-guided isolation method using silica gel column chromatography,Sephedex LH-20 chromatography,and semi-preparative HPLC.They were identified by physiochemical analysis and spectroscopic techniques as ergosta-7,9(11),22-trien-3-ol,ergosterol,spinasterol,5α,8α-epidioxy-(22E,24R)-23methylergosta-6,22-dien-3β-ol,tryptophan phenylalanine cyclic dipeptide,phenylalanine,dibutyl phthalate and diisobutyl phthalate.Only 5α,8α-epidioxy-(22E,24R)-23-methylergosta-6,22-dien-3β-ol,dibutyl phthalate and diisobutyl phthalate had strong cytotoxicity with IC50values of 14,34 μg/mL and 46 μg/mL,respectively,as evaluated by SRB method.%采用活性追踪的方法,对一株盐生海芦笋来源真菌Penicillium stoloniferum发酵产物中的抗肿瘤活性成分进行分离和鉴定。利用常压硅胶柱层析、Sephedex LH-20、半制备HPLC等分离方法从中分离得到8个单体化合物,通过理化性质分析及波谱学方法鉴定其化学结构分别为：麦角甾-7,9（11）,22-三烯-3-醇、麦角固醇、菠菜甾醇、5α,8α-环二氧-（22E,24R）-23-甲基麦角甾-6,22-二烯-3β-醇、色氨酸-苯丙氨酸-环二肽、苯丙氨酸、邻苯二甲酸正丁酯、邻苯二甲酸异丁酯;以SRB法评价化合物的抗肿瘤活性,5α,8α-环二氧-（22E、24R）-23-甲基麦角甾-6,22-二烯-3β-醇、邻苯二甲酸正丁酯、邻苯二甲酸异丁酯对小鼠乳腺癌P388细胞具有强的细胞毒活性,IC50值分别为14、34、46μg/mL,其他化合物没有活性。