Genetic fusion CONSTRUCTION FOR EXPRESSION carbohydrases multienzyme complexes in cells of filamentous fungi Penicillium verruculosum, used as the host, the mode production of recombinant fungal strains PENICILLIUM VERRUCULOSUM and methods for producing the enzyme preparation based on his

摘要

1. The genetic construct for the expression of targeted homologous and heterologous genes in cells of filamentous fungus Penicillium verruculosum, used as a host, a target comprising a coding sequence comprising a series-connected genes carbohydrases - cellobiohydrolase I, endo-1,4-β-glucanase and β II glucosidase (cellobiase) and operably linked to gene regulatory elements cellobiohydrolase I Penicillium verruculosum, which represent promoter, terminator and signal peptide gene tsellobiogidrol ABC I.2. A process for preparing a strain of the fungus Penicillium verruculosum FUS3 VKM F-4512D - producing multienzyme complex carbohydrases homologous sequences encoded by the cellobiohydrolase I, endo-1,4-β-glucanase II and a heterologous nucleotide sequence of β-glucosidase (cellobiase) Aspergillus niger, fungus comprising transforming cells Penicillium verruculosum linear genetic construct containing the target coding sequence operably linked to gene regulatory elements cellobiohydrolase I Penicillium verruculosum, which before nent a promoter, signal peptide gene and a terminator cellobiohydrolase I.3. A process for preparing multienzyme formulation given composition Penicillium verruculosum FUS3, comprising active carbohydrases that cleave vegetable polysaccharides by culturing microorganisms, characterized in that the method according to claim 2 strain obtained fungus Penicillium verruculosum FUS3 VKM F-4512D, culturing the resulting strain is carried out and the desired product is isolated from the culture medium of the fungus.