GENETIC FUSION-CONSTRUCTION FOR PROVIDING EXPRESSION OF MULTIENZYMATIC COMPLEX OF CARBOHYDRASES IN CELLS OF MYCELIUM FUNGUS Penicillium verruculosum, APPLIED AS HOST, METHOD OF OBTAINING RECOMBINANT STRAIN OF FUNGUS Penicillium verruculosum AND METHOD OF OBTAINING ENZYMATIC MEDICATION ON ITS BASE

摘要

FIELD: chemistry.;SUBSTANCE: invention relates to biotechnology, in particular to the microbiological industry, and represents a method of obtaining complex multienzymatic medications with cellobiohydrolase, endoglucanase, β-glucosidase (cellobiase) activities by the cultivation of recombinant strains of mycelium fungi of the Penicillium verruculosum family, transformed by linear fusion-construction, which represents successively connected through a linker homological genes and a heterologous gene of carbohydrases, such as cellobiohydrolase I, endogluconase and beta-glucosidase.;EFFECT: invention makes it possible to obtain effective the multienzymatic medication of a specified composition.;3 cl, 2 tbl, 3 dwg