通过在非水相中对生物催化剂脂肪酶水解大豆卵磷脂生成甘油磷酸胆碱的研究，确定最佳的工艺条件。结果表明：最佳条件为反应时间6h、反应温度40℃、pH5.0、加酶量12.5mg/mL、水含量4%，为了增加酶的利用率和减少有机溶剂的使用，反应1.5h后补加底物，补加质量为初始质量的50%，补加的底物质量浓度为0.437g/mL。在此工艺条件下，经高效液相色谱检测分析，3次平行实验测得反应液经水萃取后甘油磷酸胆碱产率为97%。该工艺为甘油磷酸胆碱的制备提供了一种可行的方法。%s:This study was undertaken to optimize reaction conditions for the biocatalytic deacylation of phosphatidylcholine (PC) by lipase to glycerol-3-phosphocholine (GPC) in non-aqueous phase. The optimal reaction conditions were found to be reaction at 40℃and pH 5.0 for 6 h with an enzyme dose of 12.5 mg/mL in the presence of 4% water. Moreover, in order to increase the utilization rate of lipase and reduce solvent consumption, additional PC at a concentration of 0.437 g/mL was added in a proportion of 50%relative to the initial amount after 1.5 h during the reaction process. Under these process conditions, the average yield of GPC after aqueous extraction of reaction products was 97%(n=3) as determined by HPLC. Thus, a feasible method to prepare GPC has been established.
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