响应曲面法优化匀浆提取银杏叶原花青素工艺

摘要

采用匀浆法提取银杏叶中的原花青素，在单因素试验的基础上，采用响应曲面法对提取过程中的工艺参数进行了优化。根据Box-Behnken中心组合试验设计原理，考察了提取溶剂、料液比、匀浆转速和匀浆提取时间等因素，以原花青素提取率为响应值进行响应面分析。响应面优化后提取工艺条件为：以无水乙醇为提取溶剂，料液比1：23.8(g/mL)、匀浆时间5.87min、转速10500r/min，在此条件下原花青素的提取率为2.21%。%This study deals with the optimization of homogenization conditions for the extraction of proanthocyanidin from Ginkgo biloba leaves using response surface methodology. The effects of extraction solvent, solid-to-solvent ratio, and homogenization speed and time on the extraction yield of proanthocyanidin was investigated by one-factor-at-a-time method. Further investigations were carried out to optimize the process parameters through response surface analysis of a mathematical model for the extraction yield of proanthocyanidin as their function built based on a Box-Behnken experimental design. The optimal conditions for extracting proanthocyanidin from Ginkgo biloba leaves were found to be homogenization at a rotary speed of 10500 r/min for 5.87 min with an absolute ethanol-to-Ginkgo biloba leaves of 1:23.8 (g/mL). Under these conditions, the extraction yield of proanthocyanidin was 2.21%.