水红花子总黄酮的提取工艺优化

摘要

分别采用乙醇回流提取法、索氏提取法、超声波辅助萃取法、水提法对水红花子总黄酮进行提取，以总黄酮提取率为指标，优选出最佳提取方法，并通过正交试验优化其提取工艺。结果表明：乙醇回流提取法具有提取率高、操作简便、成本低等优点，乙醇回流提取法的最佳工艺条件为乙醇溶液体积分数55%、料液比1：24(g/mL)、提取时间4h、提取温度90℃、提取2次，此条件下总黄酮提取率为7.06%。%Ethanol reflux extraction was selected for the extraction of total flavonoids from Fructus Polygoni Orientalis due to its advantages such as high yield, simplicity and low cost over other three extraction techniques, Soxhlet extraction, ultrasonic-assisted extraction and aqueous extraction. In order to achieve maximum extraction efficiency, the ethanol reflux extraction process was optimized using an orthogonal array design. The optimal extraction conditions were determined as two extraction cycle with 55%ethanol at a solid-to-solvent ratio of 1:24 (g/mL) and 90℃for 4 h. Under these conditions, the extraction yield of total flavonoids was 7.06%.