从绞股蓝中联合提取皂苷、黄酮和多糖工艺优化

摘要

This paper reports the application of orthogonal array design method to optimize the successive extraction of saponins, flavonoids and polysaccharides from Gynostemma pentaphyllum stems and leaves. The solvents used for the extraction of the phytochemicals were aqueous ethanol solutions at decreasing concentrations. The optimal ethanol concentration for saponins flavonoids and polysaccharides was 80%, 30%and 5%, respectively, resulting in an extraction efficiency of 8.75%, 0.75%and 3.38%, respectively. The successive extraction procedure showed more efficient utilization of raw materials than single extraction procedures and therefore could provide references for the comprehensive utilization of Gynostemma pentaphyllum.%以体积分数由高至低的乙醇溶液为单一溶剂，从绞股蓝茶叶中联合提取皂苷、黄酮和多糖的工艺，利用正交试验得到的提取多糖最优工艺条件为80%乙醇溶液提取皂苷、30%乙醇溶液提取黄酮和5%乙醇溶液提取多糖。在此条件下，皂苷提取率8.75%，黄酮提取率0.75%，多糖提取率3.38%。联合提取工艺的原料利用率高于单一提取工艺，可为绞股蓝的综合利用提供参考。