液相色谱-串联质谱法测定饮料中16种邻苯二甲酸酯

摘要

建立同时检测饮料中16种邻苯二甲酸酯的液相色谱·质谱联用检测方法。用甲醇提取饮料中的邻苯二甲酸酯，采用Aglilent Eclipse plusCls柱（2．1mm×150mm，3．5μm），以0．1％甲酸的甲醇溶液＋0．1％甲酸溶液作为流动相，梯度洗脱，质谱检测器检测，多反应监测定量。方法检出限为O．005～O．051mg／kg，回收率为89．1％～105．1％，相对标准偏差为0．8％～4．1％，可以满足饮料中邻苯二甲酸酯的检测要求。%An HPLC-ESI-MS/MS method was developed for the simultaneous determination of 16 phthalate esters (PAEs) in drinks. Methanol was used to extract PAEs in drinks. The separation was performed on an Aglilent Eclipse plus CIs (2.1 mm × 150 ram, 3.5 μ m) column with a gradient mobile phase mixture composed of water containing 0.1% fomic acid and methanol containing 0.1% fomic acid. A tandem mass detector was used for the detection and quantification of PAEs in the multiple reaction monitoring (MRM) mode. The results showed that the limit of detection of the developed method was in a range of 0.005-0.051 mg/kg. The average recovery rates of 16 PAEs across three spike levels were 89.1% - 105.1% with relative standard deviation (RSD) of 0.8% - 4.1%. This method could meet the requirements for the determination of PAEs in drinks.