脉冲强光对黑曲霉细胞膜损伤及胞内酶活性的影响

摘要

通过脉冲强光处理黑曲霉菌悬液，研究菌体细胞膜通透性的变化以及胞内酶活性的变化。结果表明：经脉冲强光处理后菌悬液的蛋白质溢出量、丙二醛的漏出量与对照相比均有显著增加，透射电子显微镜观察到细胞膜和细胞壁表面凹凸不平，出现褶皱。此外，脱氢酶活力与另外胞内3种主要抗氧化酶（超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶）活力也都有不同程度的下降。因此，霉菌屏障结构破坏所造成的细胞膜渗透压和通透性的变化可使霉菌胞内物质发生改变，甚至导致菌体死亡。%This study was designed to investigate changes in the membrane permeability and intracellular enzyme activities of Aspergillus niger before and after intense pulsed light irradiation. The results indicated that protein spillage and MDA leakage in Aspergillus niger suspension after intense pulsed light irradiation revealed an obvious increase when compared with the control group. Meanwhile, uneven and wrinkle cell membranes and walls were observed by transmission electron microscopy. Moreover, the activities of dehydrogenase and other major intracellular antioxidant enzymes also revealed a decreasing trend. Therefore, the destruction of the barrier structure of mold could result in changes in the osmotic pressure and cell membrane permeability, thus leading to changes to intracellular substances, and even cell death.